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Objective: To observe the value of a mammogram mass detection system based on deep learning (DL) in diagnosis of breast masses. Methods: Data of 298 females who underwent mammography examination were retrospectively analyzed. The reference standards of mass detection were established by three senior radiologists. The lesion detection rate and detection stability of two radiologists with working time less than 5 years were compared and analyzed without (physician 1 and physician 2) or with artificial intelligence (AI) (physician 1+AI and physician 2+AI).Results: The lesion detection rate of physician 1+AI and physician 2+AI were all higher than that of physician 1 and physician 2 (both P0.05). Conclusion: The mammogram mass detection system based on DL can effectively improve mass detection rate of junior radiologists, and enhance the robustness of detection rate of different type masses.
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Epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs) are the key to the treatment of advanced non-small cell lung cancer (NSCLC),but there are various drug-resistant mutations in the latter stage.The first and second generation of EGFR-TKIs significantly prolong the survival of patients with EGFR-activated mutant tumors.The third generation of EGFR-TKIs effectively inhibit the progress of T790M mutant tumors.The fourth generation of EGFR-TKIs inhibit both L858R,T790M and L858R,T790M,C797S mutant tumors.Using circulating tumor DNA and exogenous RNA can effectively detect the mutation types of EGFR,and choose EGFR-TKIs therapy or combined chemotherapy according to the mutation types.
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Cancer stem cells have self-renewal and multilineage differentiation potentials,which play important roles in tumorigenesis,metastasis,recurrence and resistance to chemical therapy and radiotherapy,and so on.It is the hotspot of tumor research recently.Aptamer is a small molecular oligonucleotide with high targeting ability and affinity,which is a new type of nucleic acid in the diagnosis and treatment of cancer.Now more and more research is being done on the aptamer is applied in the diagnosis and treatment of cancer stem cells,which provides a new research tool for the diagnosis and treatment of cancer stem cells.
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Nucleic acid aptamer is an oligonucleotide generated by the systematic evolution of ligands by exponential enrichment (SELEX)process from oligonucleotide library.Nucleic acid aptamer can bind to various targets with high specificity and can recognize or inhibit the biological activity of targeting molecular. Glioma-specific aptamers are developed by either targeting the glioma cells or known biomarkers,which can be coupled with nanoparticles,drugs or molecular probes,and can be applied in the imaging,targeted therapy and drug delivery of glioma.
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Objective To evaluate the effects of different analgesics combined with propofol of intravenous anesthesia on postoperative analgesia and emotion in patients of artificial abortion. Methods One hundred and twenty-two patients who had underwent painless artificial abortion were selected. The patients were divided into 4 groups by random digits table method: simple propofol group (C group, 29 cases), fentanyl combined with propofol group (F group, 30 cases), oxycodone combined with propofol group (Q group, 30 cases) and sufentanil combined with propofol group (S group, 33 cases). The induced dose of propofol was 2.5 mg/kg. When patients had limb movement during operation, a single addition of propofol 0.5 mg/kg was added until the body movement disappeared. The changes of mean arterial pressure (MAP), heart rate and respiratory rate before and after operation were compared among the 4 groups. The emotional status was assessed with affective scale before operation and 1 h after operation. The visual analog scale (VAS) was used to evaluate the degree of abdominal pain at 10, 30 and 60 min after palinesthesia. The propofol dose, operation time, recovery time and adverse reaction were recorded. Results No obvious adverse reactions were found during the operation. There was no statistical difference in operation time among 4 groups (P>0.05). The propofol dose, recovery time, body movement and the VAS score at 10, 30, 60 min after palinesthesia in F group, Q group and S group were significantly lower than those in C group, and there were statistical differences (P0.05). The MAP, heart rate and respiratory rate at beginning of the surgery and during the surgery were significantly lower than that before anesthesia in the 4 groups, and there were statistical differences (P0.05). The positive affective score after operation in C group, F group, Q group and S group was significantly higher than that before operation: (24.6 ± 5.6) scores vs. (21.7 ± 6.2) scores, (24.6 ± 3.1) scores vs. (20.6 ± 4.6) scores, (28.3 ± 6.3) scores vs. (20.8 ± 5.3) scores and (25.2 ± 5.4) scores vs. (19.9 ± 4.8) scores, and the negative affective score after operation in C group, F group, Q group and S group was significantly lower than that before operation: (17.0 ± 5.3) scores vs. (29.7 ± 7.4) scores, (17.2 ± 3.0) scores vs. (30.8 ± 5.0) scores, (16.1 ± 5.1) scores vs. (30.4 ± 4.9) scores and (17.9 ± 4.0) scores vs. (32.1 ± 5.5) scores, and there were statistical differences (P0.05). Conclusions The fentanyl, sufentanil and oxycodone combined with propofol of intravenous anesthesia in patients underwent artificial abortion can reduce propofol dose, shorten recovery time, improve positive affective score, decrease negative affective score and strengthen the analgesic effect, and doesn't increase the adverse reaction. The respiratory and circulatory inhibition effects of different analgesics combined with propofol of intravenous anesthesia were similar, but oxycodone can increase positive affective score.
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The ideal molecular imaging probe should have characteristics of specificity,sensitivity and safety.Aptamers are sing-strand DNA or RNA oligonucleotides which can bind their targets with high specificity and high affinity.Aptamers are new types of molecular imaging probe because they have many advantages,such as their large sum of possible targets,small molecule weights,easily production and modification,low immuno-genicity,high tissue penetration and so on.Now more and more aptamers research on tumor molecular imaging provides a new technology and tool for tumor diagnosis and therapy.
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Objective To explore the risk factors for methicillin-resistant Staphylococcus aureus (MRSA)infection of refractory wound,and provide reference for clinical prevention and control.Methods Clinical data of patients who were isolated Staphylococcus aureus (SA)from wound at the burn ward in a hospital from January 2006 to December 2013 were analyzed,patients were divided into four groups according to whether the isolated SA were MRSA and whether SA were from refractory wound or from non-refractory wound.Risk factors for MRSA infection of refractory wound were analyzed. Results A total of 112 isolates of SA were isolated from wound,statistical differences existed in the length of hospital stay,recent invasive operation,and recent antimicrobial use between patients in refractory wound MRSA group and refrac-tory wound methicillin-sensitive SA (MSSA)group (all P 30 days (OR 95% CI :1.14-30.69)and recent invasive operation (OR 95% CI :1.41 -17.84) were independent risk factors for refractory wound MRSA infection.There were statistically differences in previous MRSA infection,burn depth,recent operation and recent antimicrobial use between refractory wound MRSA group and non-refrac-tory wound MRSA group(all P <0.05);non-conditional logistic regression analysis revealed that recent antimicrobial use (OR 95% CI :2.080-26.800)was independent risk factor for the persistence of MRSA infection of refractory wound. Conclusion Shortening the length of hospital stay,reducing invasive operation,and using antimicrobial agents ra-tionally are helpful for the prevention and control of MRSA infection of refractory wound.
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A15, a DNA aptamer with binding specificity for U87 glioma cells stably overexpressing the epidermal growth factor receptor variant III (U87-EGFRvIII), was generated by cell systematic evolution of ligands by exponential enrichment (cell-SELEX) using a random nucleotide library. Subsequently, we established a cell enzyme-linked assay (cell-ELA) to detect the affinity of A15 compared to an EGFR antibody. We used A15 as a detection probe and cultured U87-EGFRvIII cells as targets. Our data indicate that the equilibrium dissociation constants (K(d)) for A15 were below 100 nmol/L and had similar affinity compared to an EGFR antibody for U87-EGFRvIII. We demonstrated that the cell-ELA was a useful method to determine the equilibrium dissociation constants (K(d)) of aptamers generated by cell-SELEX.
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Humanos , Aptâmeros de Nucleotídeos , Metabolismo , Neoplasias Encefálicas , Metabolismo , Linhagem Celular Tumoral , Glioma , Metabolismo , Patologia , Mutação , Ligação Proteica , Receptores ErbB , Genética , Metabolismo , Técnica de Seleção de Aptâmeros , MétodosRESUMO
Objective To investigate the characteristic of surgical treatment of burns in pediatrics patients. Methods From January 2006 to March 2012,47 pediatrics patients and 244 adult patients were received the surgical treatment in our hospital. The clinical data of two groups were analyzed retrospectively and compared. Results The female ratio in pediatric patients was higher than adult group,and almost patients received operation,especially plastic surgery and skin repair,with less surgeries of wound repair and infected surgeries. The preopera-tive physiological index between two groups was no significantly different. Conclusion On the premise of effective preoperative preparation, we could carry out surgical treatment of burns for pediatrics patients positively.
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BACKGROUND: Enthesiopathy, one of the three tough problems in sports medicine is a frequent disease in athletes;treatments to enthesiopathy determine the performance record and sports span of athletes in a big extent.DATA SOURCES: The relevant articles from 1990 to 2006 were searched for in NCBI Entrez PubMed by input the key words "enthesiopathy" and "tendinopathy" in English into a computer. Similarly, the relevant articles in Chinese from 1994 to 2006 were searched for in CNKI and Wangfang Data.STUDY SELECTION: Articles about preclinical and clinical research of enthesiopathy in athlete, the excluded articles were those about repetitive studies.DATA EXTRACTION: A total of 20 relevant literatures were collected, including 16 included articles and 4 excluded articles due to repetitive study.DATA SYNTHESIS: ① The main structure of ten-insertion which is very multiplicity contains 4 different tissues, the main function of it is to transmit and absorb shocks. ② So far it is not very clear about the etiopathogenisis and pathological change of enthesiopathy; It is considered that enthesiopathy actually is the result of decompensation in the ten-insertion and degeneration of collagen fiber and cartilaginous is the main pathological change; ③ Nonoperative therapy can abate the clinical symptom, but the curative effect is not affirmative.CONCLUSION: So far it is very clear about the anatomic structure of ten-insertion, but it is not very clear about the etiopathogenisis and pathological change of enthesiopathy and the relationship between enthesiopathy and apoptosis.Scientific and reliable therapies are scarce, strict design and random research of the existing therapies to enthesiopathy are very necessary; for importance, research and exploratory in other therapies including gene therapy and cell factor therapy is a very hopeful direction of the treatment in enthesiopathy.
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Objective To explore the curative effect and safety of CTLA-4Ig transgene for experimental autoimmune encephalomyelitis (EAE). Methods The adenovirus loaded CTLA-4Ig was injected into Wistar rat EAE models through the lateral cerebral ventricle. The clinical symptomatic and electrophysiological changes were observed to judge the efficiency of CTLA-4Ig. Results After injection of AdCTLA-4Ig into the lateral cerebral ventricle of Wistar rat EAE models, the time of EAE onset was delayed and the incidence rate was significantly reduced. And the transductive function tested by brain-stem auditory evoked potential (BAEP) and somatosensory evoked potential (SEP) was obviously improved. Conclusion CTLA-4Ig is effective in managing EAE.
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<p><b>OBJECTIVE</b>To investigate the roles of microfilaments in the expression of collagenase and TIMP-1 in normal and hypertrophic scar.</p><p><b>METHODS</b>Cell culture and Northern blot hybridization methods were used in this study.</p><p><b>RESULTS</b>After the disruption of microfilaments with cytochalasin B, mRNA contents of collagenase and TIMP-1 both increased significantly in normal and hypertrophic scar fibroblasts.</p><p><b>CONCLUSION</b>The result suggest that the microfilament cytoskeleton may regulate the synthesis and degradation of ECM, which may be a mechanism of gene regulation during wound healing.</p>
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Humanos , Citoesqueleto de Actina , Fisiologia , Células Cultivadas , Colagenases , Genética , Fibroblastos , Metabolismo , RNA Mensageiro , Inibidor Tecidual de Metaloproteinase-1 , Genética , Cicatrização , FisiologiaRESUMO
0.05). The interobserver variability in DSCT measurements was lower than in RT-3DE measurements in indexed left atrial volume and function. Conclusion DSCT can provide not only the information of coronary artery,but also highly reliable measurements of left atrial phasic volume and function at the same time.